Figure 5. Effect of miR-664b-5p on tumour growth in vivo.

(A) The gross morphology of tumours and the final xenograft tumour weights measured on day 42 after injection of tumour cells. (B) Effect of miR-664b-5p on tumour growth in vivo. Growth curves of HCC1937 subcutaneous xenograft tumours treated with miR-664b-5p or miR-NC. Tumour volumes were calculated as (long axis × short axis²)/2; n = 8 per group. (C) qRT-PCR detection of CCNE2 mRNA expression in two groups of tumour xenografts. (D) Representative images of the expression of cyclin E2, Ki67 and active caspase-3 in two groups of tumour xenografts determined by IHC. (E,F) Representative images of cyclin E2 high-expression (IHC) and miR-664b-5p low-expression (FISH) (E) or cyclin E2 low-expression and miR-664b-5p high-expression (F) in TNBC patient specimens. *p < 0.05.