Table 4. Structural deviation and solvent accessibility changes caused by ANG PD and ALS variants.
| ANG ALS/PD variant | (% RNase activity) | Solvent accessibility area of corresponding residue in ANG PD/ALS variant (Å2) | Solvent accessibility area of corresponding residue in Native ANG (Å2) | Root-mean-square deviation against native ANG** (Å) | |
|---|---|---|---|---|---|
| 1 | H13R | 0.0 | 22.7 | 9.7 | 0.27 |
| 2 | K40R | 6.3 | 79.9 | 56.8 | 0.19 |
| 3 | K54R | 48.5 | 85.7 | 81.1 | 0.26 |
| 4 | K60E | 79.6 | 135.0 | 153.9 | 0.17 |
| 5 | Q77P | 60.1 | 39.0 | 78.2 | 0.49 |
| 6 | T80S | 78.7 | 8.5 | 0.0 | 0.23 |
| 7 | R95Q | 52.0 | 123.5 | 131.6 | 0.18 |
| 8 | F100I | 39.1 | 109.7 | 104.5 | 0.31 |
| 9 | V103I | 54.1 | 0.0 | 0.1 | 0.25 |
| 10 | H114R | 1.6 | 127.0 | 87.0 | 0.44 |
| 11 | R121C | 131.2 | 58.1 | 140.9 | 0.20 |
*Percentage RNase activity to yeast tRNA in comparison to the native ANG Met−1 ANG (100%).
**Against PDB-4AOH native ANG structure by Thiyagarajan et al.8.