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. 2017 Feb 8;7:42326. doi: 10.1038/srep42326

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A,B) L. monocytogenes melt curves generated with a low imaging rate on qPCR HRM and U-dHRM platforms respectively. (C,D) L. monocytogenes melt curves generated using a high imaging rate on qPCR HRM and U-dHRM platforms respectively. The synthetic temperature calibrator sequence mean melting temperature and standard deviation are shown in all. Black circle highlights a melt curve shape feature unique to L. monocytogenes 16S sequence, which is dependent on sampling rate.