Table 1. Comparison of Genomic DNA Quanitfication Techniques.

Bacteria	Method of Quantification	Number of Genomes/μL
S. pneumoniae	Absorbance	5780
	qPCR	6554
	U-dHRM total	5460
	bacterial melt curves	1200
	non-template melt curves	4260
L. monocytogenes	Absorbance	9160
	qPCR	10839
	U-dHRM total	7580
	bacterial melt curves	2260
	non-template melt curves	5320

The concentration of genomic DNA isolated from both S. pneumoniae and L. monocytogenes was measured using an Eppendorf Biospectrometer, by qPCR standard curve method, and using U-dHRM. Total U-dHRM values are the sum of reactions identified as having specific amplification of bacterial DNA plus the reactions having off-target amplification. Reactions having no amplification, i.e. no melt curve, were classified as true negatives and make up the remainder of the 20,000 total reactions per U-dHRM chip (not represented in this table). QPCR standard curves are shown in Suppl. Fig. 2. Absorbance measurements were made on stock DNA, then the DNA was serially diluted. The calculated concentration of the dilution used on chip is reported here for each measurement modality.