Table 2. OVO SVM Classification of Mixed Genomic DNA Samples.

Experiment	Species Mixture	Absorbance		U-dHRM
		Targeted Ratio of Genomes	Estimated Number of Genomes Added to Chip	Measured Number of Genomes On-Chip	Measured Ratio of Genomes
1	S. pneumoniae		289	60
	L. monocytogenes	1:1	458	113	1: 1.88
2	S. pneumoniae		1445	238
	L. monocytogenes	3:1	458	119	2:1

DPCR chips were loaded with polymicrobial samples containing different proportions (ratios) of S. pneumoniae DNA to L. monocytogenes DNA to mimic challenging detection scenarios where one organism dominates a test sample. The targeted mixture ratios were created based on absorbance measurements of individual bacterial DNA concentrations using an Eppendorf Biospectrometer and then analyzed by U-dHRM and OVO SVM classification.