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. 2017 Feb 8;7:42111. doi: 10.1038/srep42111

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Copyright © 2017, The Author(s)

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(a) Full-length mouse integrin α5 mRNA was isolated from whole brains. Integrin α5 cDNA was amplified with PCR using specific primers. (b) Structure of the lentiviral vector expressing integrin α5 and/or Venus under the control of the EF-1α promoter. The integrin α5 gene (Itga5) was incorporated upstream of the internal ribosome entry site 2 (IRES2) region. The translation of Venus was initiated via IRES2. (c,d) Expression of Venus and integrin α5 in undifferentiated ES cells. Mouse ES cells were transfected with lentiviral vectors, and a fluorescent colony of ES cells was picked under the microscope to obtain a single clone. Scale bar = 50 μm.