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. 2017 Feb 8;7:42111. doi: 10.1038/srep42111

Figure 7. The effect of integrin α5 overexpression on the neurite outgrowth of ES cell-derived dopaminergic neurons on striatal cultures.

Figure 7

(a) Expression of Nurr1 and Ptx3 mRNA during differentiation of ES cells. ES cells transfected with lentiviral vectors were differentiated with the SDIA method for 12 days. (b) Expression of integrin α5 in differentiated ES cells. ES cells transfected with lentiviral vectors were differentiated with the SDIA method for 14 days. (c) Integrin α5 expression in TH-positive cells differentiated from LV-control transfected ES cells. Scale bar = 10 μm. (d) Venus expression in TH-positive cells differentiated from transfected ES cells. The lower images show a TuJ1-negative colony from LV-integrin α5 transfected cells. Scale bar = 200 μm. (e) The effect of integrin α5 overexpression on neural differentiation efficiency of ES cells. ES cell-derived colonies were detached from feeder cells after 14 days of differentiation and then dissociated into single cells. These cells were processed for Venus and β-III tubulin (TuJ1) immunostaining and analyzed by flow cytometry. (f) The effect of integrin α5 overexpression on dopaminergic differentiation efficiency of ES cells. (g and h) The effect of integrin α5 overexpression on the neurite length of ES cell-derived dopaminergic neurons. ES cell-derived colonies were detached from feeder cells after 13 days of differentiation and then dissociated into single cells. These cells were replated on striatal cultures and then processed for immunostaining after cultivation for the indicated times. Representative images (g) were obtained 2 days after cultivation on striatal cultures. Scale bar = 100 μm. n = 10–18. **p < 0.01 and ***p < 0.001 vs. LV-control.