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. 2017 Feb 8;7:42244. doi: 10.1038/srep42244

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Copyright © 2017, The Author(s)

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(a) The top five predicted off-target sites for mutagenesis. The mismatch (MM) sequence is labeled in red. PAM, protospacer adjacent motif. (b) PCR products flanking each predicted off-target (OT) site (#1–5) in wildtype (WT) and mutant founder (#12 and #13) mice. (c) Sub-cloning sequencing (n = 6 sub-clones/mouse) confirmed no off-target cleavage. A representative sequencing analysis of one founder (#13) is shown.