Table 1. Generation of mutant mice by Cas9 protein:crRNA:tracrRNA complex injection.

Mouse gene	No. of mice at wean	Amino acid replacement	Mutation sequence	Correct knock-in efficiency
SYNE1	17	R257H	CGG- > CAT	35%
SYNE1	9	W463D464- > AA	TGGGAC- > GCGGCC	33%
SYNE1	15	E646K	GAG- > AAG	53%
NEXN	6	R274C	CGT- > TGT	33%
NEXN	5	G645DEL	GGA- > –	40%
TAX1BP3	14	I33T	ATT- > ACT	50%
FLNC	5	S1625L	TCG- > TTG	60%
TLN	3	L432G	CTT- > GGT	67%