Table 3. Primers used in this study.
| Primer name | Sequence | GC% | Tm |
|---|---|---|---|
| ALPK2-Forward(seq) | TTAGCATATCCCATTGTGCTCATC | 42 | 62 |
| ALPK2-Reverse(seq) | AATTACTAGTGTGCTATACTGTCTAG | 35 | 61.7 |
| SYNE1-R257H-F(wt) | TGGAGGTTTCAGAAAGGACGG | 52 | 61.2 |
| SYNE1-R257H-F(mut) | TGGAGGTTTCAGAAAGGACAT | 43 | 57.5 |
| SYNE1-R257H-F(rev) | GCTAATCTTCAATTAAAATCGTCACTG | 33 | 62.1 |
| SYNE1-R257H-F(seq) | TCCCTGGGCGGTTTGGGTC | 68 | 63.6 |
| OT1-Forward(seq) | GCTGAGTGCGACTCCGTCC | 68 | 63.6 |
| OT1-Reverse(seq) | GGCTAGGTGATAAGGCTCTAGC | 55 | 64.2 |
| OT2-Forward(seq) | GTCTTTGGTCTATGTGTGATCATG | 42 | 62 |
| OT2-Reverse(seq) | CAAAGACAGGTGCAGAACAGG | 52 | 61.2 |
| OT3-Forward(seq) | GTGTAAGACATTGTGTGAAGTCCT | 42 | 62 |
| OT3-Reverse(seq) | CTGTTGCTCACAGAGTCGCCA | 57 | 63.2 |
| OT4-Forward(seq) | ATGGCATTGAAACCTTGTCAGCATA | 40 | 62.5 |
| OT4-Reverse(seq) | ACCAGCCAGTGTGTAACCAGC | 57 | 63.2 |
| OT5-Forward(seq) | GACAGTCACCGACTTGTAAAGC | 50 | 62.1 |
| OT5-Reverse(seq) | CCCTGCCACTGTGCTATCATG | 57 | 63.2 |
| PXN-L-Forward(mut) | AACGTGGTCACGAGCCCAGC | 65 | 64.6 |
| PXN-L-Reverse(mut) | GACCCTACCTATAACTTCGTATAATG | 38 | 62.9 |
| PXN-R-Forward(mut) | CATTCTTCGACCTCAGTTGAGTAG | 46 | 63.6 |
| PXN-R-Reverse(mut) | GACACACCCATAACTTCGTATAAT | 38 | 60.3 |
| PXN-Forward(seq) | TCACGGGATGAGACGACATAGG | 55 | 64.2 |
| PXN-Reverse(seq) | GTGTTCTGAGGACAGCAAGCG | 57 | 63.2 |
| PXN-CRE-Forward | TCCAGTGAGACCATGTAGTCCC | 55 | 64 |
| PXN-CRE-Reverse | GCCCTATCAAGGCAATACATCTTC | 46 | 63.6 |
ALPK, alpha kinase 2; SYNE1, nesprin-1α2; OT, off-target; PXN, paxillin, Tm, melting temperature.