Figure 6.

Activation of glutamatergic PPT neurons produces quiet wake and mild anxiety. A, After injection of CNO at 8:00 A.M., mice are mainly in quiet wake with modest amounts of time spent exploring, grooming, or nesting from 8:30 A.M. until 10:30 A.M. After injection of saline, mice are mainly asleep with a small amount of time in quiet wake during the same time interval. For comparison to injections in the light period, injections of saline in the dark period at 8:00 P.M. are associated with active wake behaviors, including exploring, grooming, and feeding from 8:30 P.M. until 10:30 P.M. (n = 4 mice). B, With open-field testing, CNO increased time in the corners compared with saline or no injection (n = 4 mice). *p < 0.05.