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Activation of cholinergic PPT neurons increases light NREM sleep. In ChAT-Cre mice, CNO (0.3 mg/kg) increases light NREM sleep and reduces deep NREM sleep for ∼3 h. The amounts of wake and REM sleep are essentially unchanged (n = 8 mice). *p < 0.05; **p < 0.01; ***p < 0.001 versus saline.
