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. 2017 Feb 8;7:40935. doi: 10.1038/srep40935

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(A) Western blot showing expression of endogenous ANO5 protein (untransfected and vector control), wild-type and mutant hANO5 (p.C356Y and p.C360Y) proteins. Mutant protein shows little or no expression. (B) Cellular localization of wild-type and mutant hANO5 proteins (red). Costaining with antibody against the calreticulin endoplasmic reticulum (ER)-specific marker (yellow) shows co-localization with the hANO5 protein (red).