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. Author manuscript; available in PMC: 2017 Feb 8.
Published in final edited form as: Anal Chem. 2016 Jan 15;88(3):1864–1870. doi: 10.1021/acs.analchem.5b04270

Figure 5.

Figure 5

Proof of concept for the prediction model for concentration along the loading portion of the PK curve. The proof of concept of the prediction model is tested by choosing a drug that has a molecular mass comparable to that of fluorescein, here levofloxacin. The desired concentration was 25.6 μM with a loading time of 1 h. The concentration in the syringes, derived from the prediction model for 1 h of flow, was 167.3 μM. Experimentally, fluorescein and levofloxacin were statistically the same, and at 1 h the concentration was within error of the theoretical concentration. Importantly, the experimental Cmax is 43.0 ± 4.1 μM. n ≥ 4; p > 0.05; error bars indicate standard deviation.