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. 2017 Feb 7;24:11. doi: 10.1186/s12929-017-0321-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Adipocytes-derived CM has no effect on the cell viability or the cell proliferation of mBMSCs. a Efficient differentiation of stromal mouse ST2 cell line into adipocytes as measured by Oil red O staining and its quantification. b Quantitative real time RT-PCR (qPCR) analysis of the adipogenic markers mRNA expression at day 12 of the adipocyte differentiation of ST2 cells. Each target gene was normalized to reference genes and represented as fold change over non-induced control cells. c Effect of CM-Adipo (100%) versus CM-Control (100%) on cell viability and (d) cell proliferation of cultured mBMSCs. Values are mean ± SD of three independent experiments, (*p < 0.05, **p < 0.005)