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. 2017 Feb 8;7:42162. doi: 10.1038/srep42162

Figure 4. CVB3 stimulation could not directly elicit ER stress in macrophages.

Figure 4

(A) GRP78 expression in RAW264.7 cells after stimulated with various doses of CVB3 for 24 h. (B) GRP78 expression in RAW264.7 cells after stimulated with CVB3 at a dose of 10 MOI for various hours. (C) GRP78 expression in BMDMs after stimulated with CVB3 at a dose of 10 MOI for various hours. (D) GRP78 expression in splenic macrophages after stimulated with CVB3 at a dose of 10 MOI in vitro for 24 h. RAW264.7 cells were infected with CVB3, inactivated CVB3 or a recombinant infectious CVB3 expressing GFP (CVB3-GFP) respectively at a dose of MOI = 10 for 24 h, and following removal of the inoculum, cells were collected and the level of CVB3 structural protein VP1 was detected by immunofluorescence assays (E), and the amounts of positive- (F) and negative-(G) strand RNAs of CVB3 were detected by real-time PCR. RAW264.7 cells, BMDMs or myocardiocytes were infected with CVB3 at 10 p.f.u. per cell and following removal of the inoculum, amounts of progeny virus were determined over 24 h (H). Individual experiment was conducted 3 times with similar results. **P < 0.01, ***P < 0.001, n.s., no significance.