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. 2017 Feb 8;7:42162. doi: 10.1038/srep42162

Figure 6. Macrophages underwent ER stress upon co-cultured with CVB3-infected, ER stressed myocardiocytes.

Figure 6

RAW264.7 cells or BMDMs were co-cultured with myocardiocytes and then infected with CVB3 (10 MOI) for 24 h, then GRP78 expression in co-cultured RAW264.7 (A) or BMDMs (B) was determined by western blot. Meanwhile, the expression of phosphorylated PERK, IRE1α and eIF2α in RAW264.7 cells (C,E,I) or BMDMs (D,F,J), and the cleavage of ATF6 in RAW264.7 cells (G) or BMDMs (H) were also detected by western blot assays. XBP1 mRNA splicing in RAW264.7 cell lines (K) or BMDMs (L) were detected by RT-PCR. Individual experiment was conducted 3 times with similar results. *P < 0.05, **P < 0.01, ***P < 0.001.