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. 2017 Feb 8;7:42162. doi: 10.1038/srep42162

Figure 7. ER stress transmission to receiver macrophages was mainly mediated by the conditioned medium of infected myocardiocytes.

Figure 7

(A) RAW264.7 and myocardiocytes were plated together or separated by trans-well membrane, infected with CVB3 (10 MOI) for 24 h, and then GRP78 expression in RAW264.7 cells was detected by western blot assays. (B) RAW264.7 cells were cultured for 6, 12 or 24 h with myocardiocyte-derived conditioned medium (c.m.) which was collected at 24 h post infection, and then GRP78 expression in RAW264.7 cells was detected by western blot assays. (C) RAW264.7 cells were cultured for 24 h with myocardiocyte-derived conditioned medium (c.m.) which was collected at various time points (6, 12 or 24 h) post infection, and then GRP78 expression in RAW264.7 cells was detected by western blot assays. Individual experiment was conducted 3 times with similar results. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significance.