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. 2017 Feb 7;14:24. doi: 10.1186/s12985-017-0688-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Typical raw fluorescence data of real-time RT-RPA assay for standard RNA in vitro transcribed from the pPPRV/RPA plasmid as they are shown in the figure. NC represent negative control. b Reproducibility of the real-time RT-RPA assay. The threshold time is represented as mean ± standard deviation (SD). The standard regression line was generated based on 6 data sets (c) Probit regression analysis using Statistics software (GraphPad Prism 5) was done on data from the six runs of real-time RT-RPA assay. The limit of detection at 95% probability is depicted by a triangle