Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 7;14:24. doi: 10.1186/s12985-017-0688-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — Determination of reaction temperature and time. a The LFS RT-RPA assay were performed respectively at different temperatures as they are shown in the figure. b After 10 min of isothermal amplification reaction, the slight test line is visible on the test strip, and when the amplification time is longer than 15 min or 20 min, the clear test line is visible on the test strip. Including the incubation time of 2 min, the whole assay time of the LFS RT-RPA assay is less than 25 min, and the results showed that this assay works effectively in a broad range of temperatures from 37 to 45 °C