Table 1.

RPA primers and probes designed in this study

Name	Sequence (5′–3′)	Genome location(X74443)
PPRV-RPA Fe1 N	GAAGAGTTCAATATGTTGTTAGCCTCCAT	588–616
PPRV-RPA Fe2 N	CCAAGGCGGTTACGGCACCGGATACGGCAGCTGAC	643–677
PPRV-RPA Fe3 N	TTACGGCACCGGATACGGCAGCTGACTCAGAACTG	652–686
PPRV-RPA Re1 N	TTTGTCAAGGCGAAATTCCCCAATCACTCTCC	715–746
PPRV-RPA Re2 N	ACTGCGTCCAGCCACCCTTTGTCAAGGCGAAATTC	729–763
PPRV-RPA Re3 N	ACTGCGTCCAGCCACCCTTTGTCAAGGCGAAA	732–763
PPRV-RPA Pe	GATACGGCAGCTGACTCAGAACTGAGAAGG (FAM-dT)G(THF)G(BHQ1-dT)TAAATACACACAACA - C3 space	663–712
PPRV-RPA Fn1 N	GAAGAGTTCAATATGTTGTTAGCCTCCAT	588–616
PPRV-RPA Fn2 N	CCAAGGCGGTTACGGCACCGGATACGGCAGCTGAC	643–677
PPRV-RPA Fn3 N	TTACGGCACCGGATACGGCAGCTGACTCAGAACTG	652–686
PPRV-RPA Rn1 N	Biotin-TTTGTCAAGGCGAAATTCCCCAATCACTCTCC	715–746
PPRV-RPA Rn2 N	Biotin-ACTGCGTCCAGCCACCCTTTGTCAAGGCGAAATTC	729–763
PPRV-RPA Rn3 N	Biotin-ACTGCGTCCAGCCACCCTTTGTCAAGGCGAAA	732–763
PPRV-RPA Pn	FAM-TACGGCAGCTGACTCAGAACTGAGAAGGTG	663–712
	-THF-GTTAAATACACACAA-C3 space

PPRV-RPA F and R, RPA primer; PPRV-RPA P, RPA Exo probe; BHQ1-dT, thymidine nucleotide carrying Black Hole Quencher 1; THF, tetrahydrofuran spacer; FAM-dT, thymidine nucleotide carrying fluorescein; C3 space, block elongation; “F”, “R”, “N”, “P”, “e” and “n” were defined as forward primer, reverse primer, nucleoprotein, probe, RPA exo kit and RPA nfo kit, respectively