Table 2.
Comparison of PPRV real-time RT-RPA assay and PPRV LFS RT-RPA assay with RT-qPCR assay on virus species, spiked samples, field samples and healthy sheep samples
| Sample type | Sample name | real-time RT-RPA(min) | LFS RT-RPA) | RT-qPCR (Cq | specific RT-qPCR(Cq) |
|---|---|---|---|---|---|
| Virus species | PPRV Nigeria 75/1 | 4.6 | + | 18 | 18 |
| GPV AV40 | - | - | - | 17 | |
| SPV Gulang2009 | - | - | - | 19 | |
| ORFV/Vaccine/CHA | - | - | - | 20 | |
| ORFV/HB/CHA | - | - | - | 22 | |
| FMDV/O/CHA | - | - | - | 21 | |
| FMDV/A/CHA | - | - | - | 19 | |
| FMDV/Asia1/CHA | - | - | - | 20 | |
| Spiked samples | Liver 1 | 4.6 | + | 16 | - |
| Liver 2 | 7.6 | + | 28 | - | |
| Liver 3 | 6 | + | 20 | - | |
| Lungs 1 | 4.3 | + | 15 | ||
| Lungs 2 | 5.3 | + | 17 | - | |
| Lungs 3 | 6 | + | 21 | - | |
| Stomach 1 | 6 | + | 23 | - | |
| Stomach 2 | 4 | + | 16 | - | |
| Stomach 3 | 5.3 | + | 18 | - | |
| Kidney 1 | 5.6 | + | 21 | - | |
| Kidney 2 | 6 | + | 24 | - | |
| Kidney 3 | 3.6 | + | 14 | - | |
| Lymphatic nodes 1 | 5.3 | + | 17 | - | |
| Lymphatic nodes 2 | 6 | + | 21 | - | |
| Lymphatic nodes 3 | 4 | + | 18 | - | |
| Skin 1 | 5.6 | + | 22 | - | |
| Skin 2 | 6 | + | 25 | - | |
| Skin 3 | 7.6 | + | 27 | - | |
| Field samples | Lung | - | - | - | - |
| Spleen | - | - | - | - | |
| Nasal swab | - | - | - | - | |
| Kidney | - | - | - | - | |
| Spleen | 6.3 | + | 25 | - | |
| Nasal swab | - | - | - | - | |
| Nasal swab | - | - | - | ||
| Kidney | - | - | 31 | - | |
| lymphatic nodes | - | - | - | - | |
| Nasal swab | 6 | + | 23 | - | |
| Kidney | - | - | - | - | |
| Spleen | 4 | + | 19 | - | |
| Nasal swab | - | - | - | - | |
| Lung | 5 | + | 19 | - | |
| Spleen | - | - | - | - | |
| Kidney | - | - | 33 | - | |
| Spleen | - | - | - | - | |
| Lung | - | - | - | - | |
| Kidney | 5 | + | 19 | - | |
| Nasal swab | 7 | + | 24 | - | |
| Spleen | 6.3 | + | 20 | - | |
| Lung | 6.6 | + | 22 | - | |
| Spleen | 5.3 | + | 20 | - | |
| Kidney | 6 | + | 20 | - | |
| Spleen | 5 | + | 18 | - | |
| Nasal swab | 7.3 | + | 26 | - | |
| Kidney | 7.6 | + | 27 | - | |
| Spleen | 5.6 | + | 21 | - | |
| Lung | 6.3 | + | 22 | - | |
| Lymphatic nodes | 7.3 | + | 27 | - | |
| Kidney | 6.6 | + | 24 | - | |
| Spleen | 6 | + | 23 | - | |
| Healthy sheep samples | Liver | - | - | - | - |
| Lungs | - | - | - | - | |
| Stomach | - | - | - | - | |
| Kidney | - | - | - | - | |
| Skin | - | - | - | - |
Samples include PPRV、sheep and goat pox viruses 、foot-and-mouth disease virus and Orf virus, eighteen spiked samples, eighteen filed samples and five healthy sheep samples. CaPV capripoxvirus, GPV goat pox viruses, SPV sheep pox viruses, CHA China. specific qPCR specific qPCR tested the respective viruses used in this study, + : positive; - : negative