Fig. 3.

a MiSeq validation of a subset of ZMEL1 mutations. A total of 384 variants were chosen, including 128 unique to Mutect or Shimmer and 128 that overlapped between the two callers. Whereas each pipeline individually only showed a 23% validation rate, this could be significantly increased to 38% by looking at the overlapping mutation calls. b Receiver-operating characteristic curves for MuTect and Shimmer show that higher MuTect scores are associated with a greater likelihood of a verified mutation, whereas the Shimmer quality scores have little relationship to validation outcome