Table 4.
Substrate | Pretreatment severity (CSF) | p-coumaric acid (mg/g lignin) | Ferulic acid (mg/g hemicellulose) | ||||
---|---|---|---|---|---|---|---|
Ester-linkeda | Ether-linkedb | Totalc | Ester-linkeda | Ether-linkedb | Totalc | ||
Miscanthus × giganteus | Untreated | 3.61 ± 0.11 | 1.20 ± 0.11 | 4.81 ± 0.08 | 1.20 ± 0.04 | 0.82 ± 0.04 | 2.02 ± 0.03 |
2.0 | 3.18 ± 0.02 | 0.33 ± 0.05 | 3.50 ± 0.05 | 0.21 ± 0.01 | 0.14 ± 0.01 | 0.35 ± 0.01 | |
2.6 | 2.84 ± 0.08 | 0.34 ± 0.08 | 3.18 ± 0.04 | 0.04 ± 0.00 | 0.06 ± 0.00 | 0.10 ± 0.01 | |
2.7 | 2.63 ± 0.04 | 0.39 ± 0.12 | 3.03 ± 0.08 | 0.05 ± 0.00 | 0.05 ± 0.00 | 0.10 ± 0.01 | |
2.8 | 2.66 ± 0.06 | 0.30 ± 0.07 | 2.96 ± 0.04 | 0.05 ± 0.00 | 0.04 ± 0.00 | 0.09 ± 0.01 | |
Wheat straw | Untreated | 0.82 ± 0.01 | 0.33 ± 0.01 | 1.15 ± 0.01 | 1.22 ± 0.01 | 0.67 ± 0.01 | 1.89 ± 0.02 |
2.0 | 0.97 ± 0.01 | 0.20 ± 0.03 | 1.18 ± 0.01 | 0.35 ± 0.03 | 0.26 ± 0.04 | 0.60 ± 0.01 | |
2.6 | 0.97 ± 0.03 | 0.24 ± 0.02 | 1.21 ± 0.01 | 0.09 ± 0.01 | 0.16 ± 0.01 | 0.26 ± 0.03 | |
2.7 | 0.96 ± 0.01 | 0.22 ± 0.01 | 1.17 ± 0.01 | 0.08 ± 0.01 | 0.14 ± 0.01 | 0.23 ± 0.01 | |
2.8 | 0.95 ± 0.02 | 0.18 ± 0.02 | 1.14 ± 0.01 | 0.08 ± 0.01 | 0.11 ± 0.01 | 0.19 ± 0.04 |
All the values are expressed as mean ± SD of triplicate
aRepresents the ester-linked fraction released during 2 M NaOH hydrolysis at 35 °C for 2 h
bRepresents the difference between the ester-linked fraction and the total fraction released during NaOH hydrolysis
cRepresents the ester- and the ether-linked fraction released during 4 M NaOH hydrolysis at 170 °C for 2 h