Fig. 3.
Laminin negatively regulates the proliferation of type I and type II pericytes. a Diagram of experimental design for Edu incorporation assay. b Immunocytochemical analysis of Edu (red) in type I pericytes isolated from wild-type (WT) and laminin γ1flox/flox:Pdgfrβ-Cre+(PKO) mice in the presence of saline (Sal) or laminin-111 (Ln). c Quantification of Edu incorporation in type I pericytes. n = 5. d Immunocytochemical analysis of Edu (red) in type II pericytes isolated from WT and PKO mice in the presence of Sal or Ln. e Quantification of Edu incorporation in type II pericytes. n = 5. f Immunohistochemical analysis of PDGFRβ (red), GFP (green), and Ki67 (magenta) expression in WT and PKO muscles. g, h Quantification of proliferating type I (g) and type II (h) pericyte number in WT and PKO mice. n = 5. Data are shown as mean ± SD. **p < 0.01 versus WT + Sal or WT; ## p < 0.01 versus PKO + Sal. Scale bars = 100 μm in b and d, and 25 μm in f