Table 2.

Sensitivity and specificity calculated for the multiplex qPCR assay, compared to the gold standard duplex PCR

Method		Total samples (n)	Target spp.	Sensitivity (%) (95% CI)	Specificity (%) (95% CI)
Group 1	multiplex qPCR	251	B.hyodysenteriae	98 (86–97)	97 (94–99)
			B.pilosicoli	93 (93–100)	96 (96–100)
Group 2	multiplex qPCR	202	B.hyodysenteriae	100 (90–100)	98 (94–100)
			B.pilosicoli	94 (87–98)	98 (94–100)
	swabsa	202	B.hyodysenteriae	87 (72–96)	98 (94–99)
			B.pilosicoli	65 (54–76)	96 (91–99)

^aSwab-coupled multiplex qPCR analysis compared with culture-coupled multiplex qPCR