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. 2017 Feb 7;17:31. doi: 10.1186/s12866-017-0943-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect on PAPI-1 transfer efficiency of the addition of OM (a) and LPS (b) preparations from PAO1 mutants lacking either CPA or OSA. Red, PAO1Δrmd (A-,B+); green, PAO1ΔalgC (A-, B+); blue, PAO1ΔwbpM (A+, B-); purple, PAO1Δwzx (A+,B-). Results were shown as mean ± SD for three independent experiments. Two-way ANOVA followed by Bonferroni post-test was used to analyze the data. All samples were compared to OM (a) or LPS (b) derived from PAO1- cells (ns: no significant difference; *, p < 0.05; **, p < 0.01; ***, p < 0.001)