Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 7;10:68. doi: 10.1186/s13071-017-2003-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 4 — Expression of rCSUI_005805. Lane M: molecular weight marker. a Analysis of expressed recombinant protein by SDS-PAGE: uninduced culture (Lane 1); induced culture with 1 mM IPTG for 0.5 h (Lane 2) and 1 h (Lane 3), arrows indicate target protein bands of ~42 kDa. b SDS-PAGE analysis of NiNTA spin column purified rCSUI_005805: elution 1 (Lane 1); elution 2 (Lane 2), arrows indicate faint protein bands of ~25 kDa; naïve merozoite lysate (Lane 3). c Batch purified rCSUI_005805 using NiNTA agarose: stepwise elution (Lanes 1–5), arrows indicate faint protein bands of ~42 kDa. d Immunoblot of batch purified rCSUI_005805 probed with anti-His HRP conjugate (Lane 1)