Fig. 5.

Analysis of adipocytes morphology and physiological condition in vitro. Porcine S/V cells were differentiated for 12 days then adipocytes were incubated with 100 μM fatty acids for 48 h. Morphology a and TAG content b in differentiated adipocytes in vitro. Medium glycerol c and FFA d released from the cells. The final albumin concentration was 1% in all culture media (i) Con (ii) LA (iii) DHA (iv) OA (Con = control, no exogenous FA added, LA = linoleic acid (C18:2n-6), DHA = docosahexaenoic acid (C22:6n-3) and OA = oleic acid (C18:1n-9). Images were taken at 200× magnification using phase contrast microscopy. Primary porcine adipocytes were incubated with 100 μM fatty acids for 48 h. N = 6 independent experiments, each with cells isolated from a different pig. Analysis of variance, followed by Tukey’s post-hoc test was used to determine statistic significances. Bars with different letters were statistically significant (p ≤ 0.05)