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. 2017 Feb 8;7:42186. doi: 10.1038/srep42186

Figure 5. Neutralization of TGF-β attenuates tumour extravasation and pulmonary metastasis.

Figure 5

(a) Mouse control IgG (Mouse IgG) or TGFβ neutralizing mAb (clone 1D11) (100 μg/mouse) was administrated by the intravenous (i.v.) route to 5-week-old male CB17/Icr-Prkdcscid/CrlCrlj mice 1 h before i.v. inoculation of UM-UC-5 cell suspensions (1.0 × 106/mouse). In some experiments, 1D11 mAb was administrated 2 and 4 days after tumor cell inoculation (1D11 mAb (x3)). Mice were euthanized 30 days after cell inoculation and metastatic foci on the lung surface were counted. Bars represent mean values. **P < 0.01 by the Mann-Whitney U test. (b) Mouse control IgG (Mouse IgG) or TGFβ neutralizing mAb (clone 1D11) (100 μg/mouse) was administrated to 5-week-old male CB17/Icr-Prkdcscid/CrlCrlj mice 1 h before tumour inoculation. Calcein-AM-labeled UM-UC-5 cell suspensions were then inoculated (1.0 × 106/mouse) into mice. After 30 min or 48 h after i.v. tumour inoculation, mice were euthanized and frozen lung sections were fixed and stained by Hoechst 33342. Representative merged images of calcein-AM-labeled UM-UC-5 cells (green) stained for nuclear DNA (blue) are shown. (c) The fluorescence intensity of calcein-AM was measured using BioRevo BZ-9000. N.S., not significant. *P < 0.05 by the Mann-Whitney U test.