Figure 4. Size Exclusion Chromatography of BmrA in complex with various detergents.

BmrA was purified with FC12, DDM and LMNG on IMAC, from which the concentrated pools were concentrated and submitted to SEC. BmrA was probed by its absorbance at 280 nm (green) and detergents quantified by MALDI-TOF MS (blue). The same was done without BmrA (absorbance in orange trace and detergent amounts in red diamonds). Quantifications were done in triplicate on the same experiment.