Table 1. Quantification of bound detergents to membrane proteins.
| Proteins | Det. | step | [Prot] (μM) | Cor.[Det] (mM) | Det./Prot. (mol/mol) | |
|---|---|---|---|---|---|---|
| SERCA1a (10 TMS) | Monomer 994 res., 109.5 kDa | DDM | SEC | 6.03 | 0.934 | 155 ± 27 |
| BmrA (2 × 6 TMS) | Homodimer 2 × 590 res. 130 kDa | FC12 | IMAC | 12.3 | 5.7 | 463 ± 18 |
| IMAC | 2.7 | 1.2 | 457 ± 17 | |||
| SEC | 8.4 | 4.9 | 589 ± 60 | |||
| DDM | IMAC | 7.5 | 2.5 | 328 ± 8 | ||
| SEC (peak average) | 9.5 | 3.7 | 412 ± 40 | |||
| SEC | 11.9 | 1.6 | 399 ± 30 | |||
| LMNG | IMAC (pool) | 2.5 | 0.5 | 176 ± 28 | ||
| IMAC | 4.4 | 0.8 | 138 ± 11 | |||
| SEC (peak average) | 4.4 | 0.9 | 157 ± 13 | |||
| SEC | 5.9 | 0.9 | 167 ± 10 | |||
| BmrCD (6 + 6 TMS) | Heterodimer 585 + 673 res. 138.4 kDa | DDM | IMAC | 34.5 | 15.9 | 459 ± 12 |
| SEC | 2.5 | 1.1 | 444 ± 12 | |||
| LacY (12 TMS) | Monomer 417 res., 47.2 kDa | DDM | SEC | 12.2 | 4.7 | 383 ± 27 |
| hAAC1 (6 TMS) | Monomer 298 res., 33 KDa | FC12 | SEC | 27.0 | 4.3 | 157 ± 22 174 ± 10 |
| OprM (β barrel) | Trimer 3 × 474 res. 156.3 kDa | DDM | SEC | 38.0 | 11.4 | 300 ± 6 |
| OG | SEC | 4.84 | 1.77 | 364 ± 70 | ||
| hP2Y1r (7 TMS) | Monomer 268 res., 30.3 kDa | DDM | Desalting | 27.8 | 6.2 | 223 ± 6 |
SERCA1a is the Ca2+-transporting ATPase from rabbit, BmrA and BmrCD54 are ABC transporters from Bacillus subtilis, LacY55 is the lactose transporter of E. coli, hAAC156 is the human ADP/ATP carrier, OprM57 is a component of the MexA-B/OprM drug efflux system of Pseudomonas æroginosa, hP2Y1r58 is the human purinergic receptor. Proteins were purified by IMAC and SEC as indicated (Methods) and detergents quantified. When not indicated, the SEC or IMAC fraction tested corresponds to the peak head. Quantifications were done in triplicate on the same experiment.