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. 2016 Dec 12;9(2):257–268. doi: 10.1080/19420862.2016.1267090

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Figure 5. — Purification using AEX of BsAbs that could not be purified to homogeneity using CEX. (A) Purification of BsAb#6 using a Mono Q 10/100GL column and the CEX buffer system with a pH gradient from pH 9.17 to 8.21 (B) Purification of BsAb#6 using a Mono Q 10/100GL column and the AEX buffer system with a pH gradient from pH 9.34 to 7.51 (C) Secondary purification of the fractions of BsAb#7 highlighted in Fig. 3F using a Mono Q 10/100GL column and a pH gradient from pH 9.57 to 8.07 (Note: the load amount was 4.4 mg).