Figure 4.

Binding response levels of full-length rhumAb (in blue) and the F(ab’)2 (in red) of rhumAb binding to immobilized recombinant rat, cynomolgus monkey, and human FcRn. (A) at acidic pH (pH = 6.0); (B) at physiological pH (pH = 7.4). Concentration of tested full-length and F(ab’)2 of rhumAb samples is 5 µM. The in-line reference subtracted response was corrected by buffer sample, and was reported 5 seconds before the end of each injection. The reported data was averaged from 2 individual experimental runs. The experiments were conducted using running buffer containing PBS, 0.05% polysorbate 20, pH 6.0 or pH 7.4.