Figure 5.

Binding response levels of the full-length molecules and F(ab’)2 of rhumAbs to Immobilized recombinant rat, cynomolgus monkey, and human FcRn in the presence of additional salt (0–0.5 M) in running buffers (PBS, 0.05% polysorbate 20, pH 6.0 or pH 7.4) (A) at acidic pH (pH = 6.0); (B) at physiological pH (pH = 7.4). F(ab’)2 of rhumAbs were only tested only in PBS buffers with and without additional 0.1 M NaCl. Concentration of tested the full-length molecules and F(ab’)2 of rhumAb samples is 5 µM. The in-line reference subtracted response was corrected by buffer sample, and was reported 5 seconds before the end of each injection.