Figure 6.

Binding response levels of wild type rhumAb2 and its variants to immobilized recombinant rat FcRn at acidic pH (pH 6.0). Concentration of tested rhumAb is 3 nM. The in-line reference subtracted response was corrected by buffer sample, and was reported 5 seconds before the end of each injection. The reported data was averaged from 2 individual experimental runs. The experiments were conducted using running buffers containing PBS, 0.05% polysorbate 20, pH 6.0.