Table 3.
Binding response of recombinant rat FcRn rhumAbs on reference and test flow cells (FCs) in assay formats 1 and 2 at pH 6.0.
| Format 1 (ligand: rhumAb; analyte: recombinant rat FcRn) |
Format 2 (ligand: recombinant rat FcRn; analyte: rhumAb) |
|||||
|---|---|---|---|---|---|---|
| Reference FC (RU) | rhumAb immobilized FC (RU) | rhumAb immobilized FC with reference FC -signal subtracted (RU) | Reference FC (RU) | rat FcRn immobilized FC (RU) | rat FcRn immobilized FC with reference FC signal subtracted (RU) | |
| rhumAb1 | 36 | 63 | 27 | 261 | 302 | 41 |
| rhumAb2 | 36 | 82 | 46 | 924 | 1634 | 710 |
Note: Each binding response was reported 5 seconds before the end of each injection. All data were corrected after the in-line reference subtraction followed by buffer sample subtraction. In format 1, the concentration of recombinant rat FcRn is 5 µM. In format 2, the concentration of the rhumAb is 5 µM. Binding of rhumAbs to recombinant cynomolgus monkey and human FcRn proteins had the similar trends, and therefore are not shown here.