Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 8;9(2):213–230. doi: 10.1080/19420862.2016.1267089

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

PMC Copyright notice

Figure 1. — Computational design and selection of HC and LC variants. (A) Open book view of human IgG₁ C_H1/C_L domains (PDB 1N8Z)⁵⁷ showing side-chains of domain interface residues selected for Design 1. Residues C_L S176 and C_H1 F170 were selected for design of knob and hole mutations, respectively. (B) Open book view of human IgG₁ C_H1/C_L domains (PDB 1N8Z) showing side-chains of domain interface residues selected for Design 2. Residues C_H1 L128 and C_L F118 were selected for design of the first knob and hole pair, respectively, and residues C_L L135 and C_H1 V185 for the second knob and hole pair, respectively (C) Energy contrast filter for computational designs. (D) Contrast vs. stability plot for the unique sequence outputs from Rosetta. Filled and unfilled symbols correspond to variants that were or were not evaluated in experiments, respectively.