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. 2016 Dec 8;9(2):213–230. doi: 10.1080/19420862.2016.1267089

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© 2017 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — Identification of charge pairs in IgG₁ constant and variable domains. (A) Effect of single and double C_L domain charge variants upon expression. (B) Effect on C_L mutations on antigen binding as assessed by bacterial display.²⁹ (C) Purification yield of constant domain charge pair variants. (D) Purification yield of variable domain charge pair variants. Data shown (A, C, D) are mean values ± SD.