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Dose response plots of the whole peptide 259TPEVTCVVVDVSHEDPEVK (A), or individual residues E261 (B), D268 (C), E272 (D) in intact or deglycosylated mAb-A. Black color represents the intact mAb and red color represents deglycosylated mAb-A and each data point is an average of three experiments. The solid lines show the best fit to first-order exponential equation on which the RC of the peptide or individual residues was determined and shown on the right.
