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. 2016 Dec 22;18(1):1. doi: 10.3390/ijms18010001

Figure 2.

Figure 2

CD8+ T cell activation requires an inflammatory microenvironment. CD4+ T cells (CD45+ CD3+ CD4+ CD8−) and CD8+ T cells (CD45+ CD3+ CD4− CD8+) were isolated from obstructed kidney or spleen. (A–D) mRNA levels of chemokines (CCL2, CCL3, CCL4, and CCL5) as determined in those CD4+ T cells or CD8+ T cells by quantitative RT-PCR (qRT-PCR; * p < 0.05 kidney vs. spleen; n = 5); (E–H) mRNA levels of cytotoxicity-related genes (PRE-1, GZMA, GZMB, and FASL) as determined in those CD4+ T cells or CD8+ T cells by qRT-PCR (* p < 0.05 kidney vs. spleen; n = 5).