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. 2016 Dec 22;18(1):1. doi: 10.3390/ijms18010001

Figure 6.

Figure 6

CD11c+ CD8+ T cells isolated from obstructed kidney can induce fibroblast death in vitro. (A) Fibroblasts were co-cultured with CD11c− CD8+ T cells or CD11c+ CD8+ T cells for 24 h; the cells were subsequently harvested to analyze the mortality of CD8− CD3− cells (fibroblast) through PI (Propidium Iodide) staining combined with flow cytometry; the CD8+ T cell-free set-up served as control; (B) Quantitative analysis of the percentage of PI staining-positive fibroblasts. (* p < 0.05 vs. fibroblasts plus CD11c− CD8+ T cells; n = 5/group).