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. 2017 Jan 17;18(1):3. doi: 10.3390/ijms18010003

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of lentiviral-mediated shRNAi on HIF-1α mRNA expression and protein accumulation under four-week hypoperfusion. (A,B) Western blot analysis of HIF-1α protein levels in hippocampus of 2-vessel occlusions (2VO) or sham rats that received stereotaxic injection of pLVx-GFP-shCon or pLVx-GFP-shHIF-1α. As blank control, 2VO or sham rats without stereotaxic injection were also included in our study; (C) qRT-PCR analysis of HIF-1α mRNA levels in hippocampus of rats in each group; (D,E) Western blot analysis of HIF-1α protein levels in cortex of rats in each group; (F) qRT-PCR analysis of HIF-1α mRNA levels in cortex of rats in each group. Values for HIF-1α protein and mRNA were normalized to β-actin protein or mRNA, respectively. Values are expressed as a percentage of the average value for the first group on the left and are means ± SEM (n = 4–5). * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA followed by Bonferroni post hoc tests).

The effects of lentiviral-mediated shRNAi on HIF-1α mRNA expression and protein accumulation under four-week hypoperfusion. (A,B) Western blot analysis of HIF-1α protein levels in hippocampus of 2-vessel occlusions (2VO) or sham rats that received stereotaxic injection of pLVx-GFP-shCon or pLVx-GFP-shHIF-1α. As blank control, 2VO or sham rats without stereotaxic injection were also included in our study; (C) qRT-PCR analysis of HIF-1α mRNA levels in hippocampus of rats in each group; (D,E) Western blot analysis of HIF-1α protein levels in cortex of rats in each group; (F) qRT-PCR analysis of HIF-1α mRNA levels in cortex of rats in each group. Values for HIF-1α protein and mRNA were normalized to β-actin protein or mRNA, respectively. Values are expressed as a percentage of the average value for the first group on the left and are means ± SEM (n = 4–5). * p < 0.05 and ** p < 0.01, *** p < 0.001 (one-way ANOVA followed by Bonferroni post hoc tests).