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. 2017 Jan 17;18(1):3. doi: 10.3390/ijms18010003

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of HIF-1α knockdown on CCH-induced glial activation. Representative photomicrographs of glial fibrillary acidic protein (GFAP) (A) and Iba-1 (B) immunohistochemistry in cortex, corpus callosum, and four subregions of the hippocampus four weeks after sham or 2VO surgery with or without HIF-1α-knockdown treatment. The positive cells are dyed brown. Bar = 50 µm (the magnification of all images is consistent). The Histograms showing the densities of the cells that were immunoreactive for GFAP (A) and Iba-1 (B) (per mm²). The bars represent the mean ± SEM (n = 8). * p < 0.05, ** p < 0.01 (One-way ANOVA followed by Bonferroni post hoc tests).

The effects of HIF-1α knockdown on CCH-induced glial activation. Representative photomicrographs of glial fibrillary acidic protein (GFAP) (A) and Iba-1 (B) immunohistochemistry in cortex, corpus callosum, and four subregions of the hippocampus four weeks after sham or 2VO surgery with or without HIF-1α-knockdown treatment. The positive cells are dyed brown. Bar = 50 µm (the magnification of all images is consistent). The Histograms showing the densities of the cells that were immunoreactive for GFAP (A) and Iba-1 (B) (per mm²). The bars represent the mean ± SEM (n = 8). * p < 0.05, ** p < 0.01 (One-way ANOVA followed by Bonferroni post hoc tests).