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. 2017 Jan 17;18(1):3. doi: 10.3390/ijms18010003

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of HIF-1α knockdown on the expression of downstream genes during CCH. (A,B) Western blot analysis of VEGF/GLUT-1/EPO protein levels in hippocampus four weeks following 2VO or sham operation with or without HIF-1α knockdown; (C) qRT-PCR analysis of VEGF/GLUT-1/EPO and Noxa/Nix/Binp-3mRNA levels in hippocampus of rats in each group. Values for protein and mRNA were normalized to β-actin protein or mRNA, respectively. Values are expressed as a percentage of the average value for sham + pLVx-GFP-shCon rats and are mean ± SEM (n = 4–5). * p < 0.05 (One-way ANOVA followed by Bonferroni post hoc tests).