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. 2016 Dec 23;18(1):26. doi: 10.3390/ijms18010026

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunostaining for GSK3β and p-GSK3β. CTR-GMSCs showed positive staining for both GSK3β and p-GSK3β, whereas CBD-GMSCs showed negative expression. GMSCs pre-treated with Capsazepine/TRPV1 antagonist (CAPSZ-GMSCs) were positive for GSK3β and p-GSK3β; CTR-GMSCs vs. CBD-GMSCs **** p < 0.0001; CBD-GMSCs vs. CAPSZ-GMSCs **** p < 0.0001. Densitometric analysis was carried out on 3/6 coverslips by covering about 90% of the total area for each experimental group. Objective: 40×, bar length: 50 μm.