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. 2016 Dec 27;18(1):38. doi: 10.3390/ijms18010038

Figure 3.

Figure 3

Effects of MIA and/or vitamin C on apoptosis induction and cell cycle progression. SW1353 cells were incubated with 100 μM vitamin C in the presence or absence of 5 μM MIA for 24 h, and then (A) apoptosis was determined by Hoechst 33342 staining; and (B) the distribution of cells in the different phases of the cell cycle was determined by flow cytometry. In (A), the white arrows indicate an apoptotic cell; in (B), the down panel indicates the percentage of cell cycle distribution. The results are expressed as the mean ± S.D. for three separate experiments, each in triplicate. * p < 0.05 compared to the untreated control. # p < 0.05 compared to the MIA treated group.