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Western-blot analysis of SMG1, D1 and SAPK9 to verify the Mass Spectrometry (MS)-identified phosphorylation pattern at CK, 3 and 12 h. Red triangle indicates the target band in original size; Red asterisk indicates the phosphorylated target protein band. Anti-tubulin was used as an internal control for normalization. The values above the phosphorylated bands represent the normalized, relative band intensities by setting CK to 1.
