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. 2017 Jan 4;18(1):87. doi: 10.3390/ijms18010087

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The immunofluorescence staining of chondrogenic- or osteogenic-related proteins to verify phenotypic functions of chondrocytes labeled by 250 µg/mL magnetic nanoparticles. Chondrocytes stained for nuclei (blue) and α-SMA (green) to show cell morphology (A). Cells stained with chondrogenic related antibodies to collagen type II (B), aggrecan (C), and Sox-9 (D). Stained with osteogenic related antibodiesMMP-13 (E), osteonectin (F), and the negative control (G). Green indicates specific antibodies, blue, the nucleus.