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. 2017 Jan 6;18(1):109. doi: 10.3390/ijms18010109

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© 2017 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bone sample characterization. (A) Microscopy image (hematoxylin and eosin staining) showing the features typical of bone tissue and inflammatory infiltration; example of a laser captured microdissected area of the bone sample: (B) shows the pre-microdissected area (40× resolution); (C) the green line encircles the microdissected area; (D) sex-determining region Y amplification revealed the sex of the patient: lane 1: ladder (100–1200 bp); lane 2: sample; lane 3: negative control; lanes 4 and 5: male controls; lanes 6–8: uncharged slots.